Device

Part:BBa_K4061200:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-16)


Scaffold gene kit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 214
    Illegal SapI.rc site found at 374


Design Notes

The design considerations for each part involved could be seen at their respective pages. This device compiles these parts. We used constitutive promoter to drive the expression of both engineered proteins (proteins with modified domains) while kept the scaffold under an inducible promoter. Teams however needed to visit each part (protein domain) and attach the domain themselves next to their protein CDS while designing their parts.


Source

Whitaker, W. R. et al. "Engineering Robust Control Of Two-Component System Phosphotransfer Using Modular Scaffolds". Proceedings Of The National Academy Of Sciences, vol 109, no. 44, 2012, pp. 18090-18095. Proceedings Of The National Academy Of Sciences, https://doi.org/10.1073/pnas.1209230109.

References